Studies on the method of measuring the enzyme activity of feed xylanase
Author(s): FEI Dibo, FENG Guanquan, YUAN Chao
Pages: 53-58
Year: 2004 Issue: 2
Journal: ACTA AGRICULTURAE ZHEJIANGENSIS
Keyword: 饲用木聚糖酶; 测定方法; DNS法;
Abstract: 探讨了木聚糖酶活测定中的几个关键因素对酶活测值的影响,并以此为主要依据,建立了应用DNS法测定饲用木聚糖酶活力的方法:在40℃,pH 5.0条件下,底物浓度1%,酶促反应时间10 min, DNS显色5 min,于波长540～550 nm处测定吸光度A.
Pages: 53-58
Year: 2004 Issue: 2
Journal: ACTA AGRICULTURAE ZHEJIANGENSIS
Keyword: 饲用木聚糖酶; 测定方法; DNS法;
Abstract: 探讨了木聚糖酶活测定中的几个关键因素对酶活测值的影响,并以此为主要依据,建立了应用DNS法测定饲用木聚糖酶活力的方法:在40℃,pH 5.0条件下,底物浓度1%,酶促反应时间10 min, DNS显色5 min,于波长540～550 nm处测定吸光度A.
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