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Preparation of ditags in constructing SAGE library of Monasccus Ruber
Author(s): 
Pages: 19-22
Year: Issue:  1
Journal: CHINESE JOURNAL OF ECO-AGRICULTURE

Keyword:  红曲霉SAGE文库双标签;
Abstract: 研究探讨了红曲霉SAGE文库构建过程中总RNA提取、mRNA分离纯化、cDNA合成以及双标签(Ditags)制备方法,并通过PCR条件的优化获得双标签PCR的最佳模板稀释浓度及扩增循环数.
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