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Retrovirus-mediated transfer of GFP gene into lens epithelial cells in vitro
Author(s):
WAN Guangming
,
ZHANG Xiaofang
Pages:
34
-
36
Year:
2004
Issue:
1
Journal:
CHINESE OPHTHALMIC RESEARCH
Keyword:
晶状体上皮细胞
;
后发性白内障
;
报告基因
;
绿色荧光蛋白
;
基因治疗
;
Abstract:
目的观察目的基因在晶状体上皮细胞的表达情况,以探讨基因疗法用于后发性白内障的可行性.方法用绿色荧光蛋白基因作为报告基因,以逆转录病毒载体携之转染晶状体上皮细胞,观察GFP在晶状体上皮细胞的表达情况.结果基因转染后可见晶状体上皮细胞的GFP表达率在30%~35%.结论逆转录病毒载体可以携带目的基因转染于晶状体上皮细胞,并达到较高的转染率.
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