Enantiomeric separating study of D-and L-carnitine with capillary zone electrophoresis
Author(s): LENG Taohua, GUO Weiqiang, REN Yiping
Pages: 105-108
Year: 2003 Issue: z1
Journal: JOURNAL OF ZHEJIANG UNIVERSITY OF SCIENCE AND TECHNOLOGY
Keyword: derivatization; enantiomeric separation; electrophoresis; carnitine;
Abstract: A new capillary zone electrophoretic method has been developed for the enantiomeric separation and quantification of enantiomers of carnitine. D-and L-carnitine were derivatized with 4'-bromophenacyl bromide producing a derivatization that can be detected by UV absorbance detection. The separation was performedby using a selective chiral containing sulfated-β-cyclodextrin and (S, S)-1,7-bis(4-benzyl-5-hydroxy-2-oxo-3-azapentyl)-1,7-diaza-12-crown-4. Acetonitrile was used as electroosmotic modifier and separation was carried out in an uncoated capillary. Under the optimal conditions D-and L-carnitine were separated completely and the limits of detection for both isomers were about 80 ng/mL.
Pages: 105-108
Year: 2003 Issue: z1
Journal: JOURNAL OF ZHEJIANG UNIVERSITY OF SCIENCE AND TECHNOLOGY
Keyword: derivatization; enantiomeric separation; electrophoresis; carnitine;
Abstract: A new capillary zone electrophoretic method has been developed for the enantiomeric separation and quantification of enantiomers of carnitine. D-and L-carnitine were derivatized with 4'-bromophenacyl bromide producing a derivatization that can be detected by UV absorbance detection. The separation was performedby using a selective chiral containing sulfated-β-cyclodextrin and (S, S)-1,7-bis(4-benzyl-5-hydroxy-2-oxo-3-azapentyl)-1,7-diaza-12-crown-4. Acetonitrile was used as electroosmotic modifier and separation was carried out in an uncoated capillary. Under the optimal conditions D-and L-carnitine were separated completely and the limits of detection for both isomers were about 80 ng/mL.
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