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High expression of fusion epitopes cDNA of foot-and-mouth disease virus in pET proeukaryotic system and purification of products
Author(s): CHEN Zuhuan, ZHANG Hongying, GUO Zuojun, LIN Zuo, ZHU Weijia, SUN Shuhan
Pages: 904-
905
Year: 2003
Issue:
8
Journal: ACADEMIC JOURNAL OF SECOND MILITARY MEDICAL UNIVERSITY
Keyword: 口蹄疫病毒; 表位; 基因表达; pET原核系统;
Abstract: 构建口蹄疫融合表位基因-SG表达载体pET-SG,转化重组子与K802,新鲜过夜菌以2%接种量接种2YT培养液,37℃培养2 h后用IPTG诱导,每隔1 h取样,共8 h.根据SDS-PAGE结果,口蹄疫融合表位基因-SG在pET原核表达系统可获得较高表达,用IPTG诱导4 h蛋白表达量最高,用Ni+柱亲和层析可得到较纯蛋白.
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