The server is under maintenance between 08:00 to 12:00 (GMT+08:00), and please visit
later.
We apologize for any inconvenience caused
Characterization of programmed cell death 5 (PDCD5) gene in human cartilage and its possible significance
Author(s): CHENG Aixin, WANG Zuo, MA Dalong, ZHOU Zuozuo, LOU Siquan
Pages: 481-
484
Year: 2003
Issue:
5
Journal: JOURNAL OF PEKING UNIVERSITY(HEALTH SCIENCES)
Keyword: PDCD5; 骨关节炎; 软骨; 关节;
Abstract: 目的:研究程序化死亡基因5 (PDCD5)在正常及骨关节炎关节软骨中的表达规律,探讨PDCD5在骨关节炎发病中的作用.方法:取20例骨关节炎及10例股骨颈骨折行关节置换术时切除的关节软骨,分离软骨细胞,留取组织块制作石蜡切片.分别用流式细胞术、直接免疫荧光、激光共聚焦显微镜及RT-PCR检测PDCD5在正常及骨关节炎软骨细胞内的表达水平及部位,免疫组织化学检测PDCD5在关节软骨内的表达特征.结果:在骨关节炎软骨细胞中PDCD5表达上调,以细胞核内增强为主;在骨关节炎的组织切片中PDCD5表达增强,阳性细胞主要分布在表层及深层,而在股骨颈骨折中PDCD5阳性细胞主要分布在表层及中层.结论:PDCD5可能参与了骨关节炎软骨细胞的凋亡过程,在骨关节炎的发病中发挥作用.
Citations
No citation found