The server is under maintenance between 08:00 to 12:00 (GMT+08:00), and please visit later.
We apologize for any inconvenience caused
Login  | Sign Up  |  Oriprobe Inc. Feed
China/Asia On Demand
Journal Articles
Laws/Policies/Regulations
Companies/Products
Bookmark and Share
Alteration of the expression of cartilage matrix molecule in the cartilage by the chondrocytes from rabbit temporomandibular joint with experimental osteoarthritis and its response to interleukin-1β
Author(s): 
Pages: 521-524
Year: Issue:  5
Journal: JOURNAL OF PEKING UNIVERSITY(HEALTH SCIENCES)

Keyword:  白细胞介素1β颞下颌关节疾病软骨细胞/代谢细胞生物学;
Abstract: 目的:研究外源性炎症因子白细胞介素1β(interleukin-1β, IL-1β)对颞下颌关节骨关节病与正常髁突软骨细胞代谢活动的影响,探讨其在颞下颌关节骨关节病进展过程中所发挥的作用. 方法:采用20 μg*L-1重组白介素1β(recombined human interleukin-1β, rhIL-1β)刺激体外贴壁培养条件下的成兔正常成熟髁突软骨细胞与兔实验性颞下颌关节骨关节病模型髁突软骨细胞, RT-PCR方法检测、比较细胞对软骨基质成分Ⅱ型胶原、蛋白多糖聚糖体、胶原酶以及内源性生长因子胰岛素样生长因子1(insulin-like growth factor-1, IGF-1)和转移生长因子β1(transforming growth factor-β1, TGF-β1)的mRNA表达变化.结果: 在20 μg*L-1 rhIL-1β的刺激下,(1)正常成熟髁突软骨细胞Ⅱ型胶原和软骨蛋白多糖聚糖体的表达均明显降低,胶原酶表达水平变化不明显;(2)在IL-1β的作用下,骨关节病软骨细胞对Ⅱ型胶原和胶原酶的表达水平降低,对软骨蛋白多糖聚糖体的表达水平无明显影响;(3)IL-1β对正常和骨关节病髁突软骨细胞内源性生长因子IGF-1和TGF-β1的表达均无明显影响.结论: 蛋白多糖聚糖体的合成,导致髁突软骨病变发生,而且能不断干扰骨关节病髁突软骨细胞代谢,导致关节软骨基质环境进一步恶化.
Related Articles
No related articles found