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Molecular detection of dapsone and rifampicin resistance to Mycobacterium leprae in newly detected and relapsed patients
Author(s): 
Pages: 89-92
Year: Issue:  2
Journal: CHINA JOURNAL OF LEPROSY AND SKIN DISEASES

Keyword:  麻风病复发氨苯砜利福平耐药麻风菌;
Abstract: 目的:检测麻风菌的氨苯砜、利福平耐药基因,以了解复发与耐药的关系.方法:在PCR扩增出麻风菌的氨苯砜耐药基因fol P1、利福平耐药基因rpoB靶片段的基础上,采用直接测序和异源双链分析确定相应基因是否有突变.结果:在7例复发病例中,发现2例为DDS耐药菌株,但未发现有RFP耐药菌株.在35例新病人中,有22例和31例分别扩增出fol P1和rpoB基因,但均未发现有DDS、RFP基因突变.结论:结合2例DDS耐药病例的临床病史分析提示,fol P1基因不同突变,可能导致不同程度的DDS耐药.虽然本研究未发现有RFP耐药菌株,但是对复发病例开展DDS、RFP耐药检测却是十分必要的.
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