Shedding virus and rapid propagation of Solanum muricatum Ait
Author(s): LAN Wei, LIANG Jun
Pages: 30-33
Year: 2003 Issue: 5
Journal: JOURNAL OF BIOLOGY
Keyword: 人参果; 脱毒; 快速繁殖;
Abstract: 用人参果无菌实生苗茎尖进行组织培养,经过5代高温加剥离茎尖培养的方法,可以完全脱除病毒.无菌小苗茎尖接种在MS+6-BA1.0mg/L+N从0.1mg/L+2.4-D0.10mg/L的培养基上能够产生淡黄色愈伤组织,继续培养分化出较多芽点;把带芽点的愈伤组织转移至MS+6-BA1.0mg/+LAA0.05mg/L的培养上可分化出丛生芽且继代增殖效果较好;丛生芽在1/2MS+NAA0.1mg/L+PP3330.2mg/L的培养基上易生根,可以获得健壮完整的小苗.
Pages: 30-33
Year: 2003 Issue: 5
Journal: JOURNAL OF BIOLOGY
Keyword: 人参果; 脱毒; 快速繁殖;
Abstract: 用人参果无菌实生苗茎尖进行组织培养,经过5代高温加剥离茎尖培养的方法,可以完全脱除病毒.无菌小苗茎尖接种在MS+6-BA1.0mg/L+N从0.1mg/L+2.4-D0.10mg/L的培养基上能够产生淡黄色愈伤组织,继续培养分化出较多芽点;把带芽点的愈伤组织转移至MS+6-BA1.0mg/+LAA0.05mg/L的培养上可分化出丛生芽且继代增殖效果较好;丛生芽在1/2MS+NAA0.1mg/L+PP3330.2mg/L的培养基上易生根,可以获得健壮完整的小苗.
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