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The Method of Rapid Determination of Serum Arginine
Author(s): 
Pages: 61-62
Year: Issue:  4
Journal: AMINO ACIDS AND BIOTIC RESOURCES

Keyword:  精氨酸氨基酸分析仪快速检测血清;
Abstract: 建立快速、准确的精氨酸定量检测方法.采用6300黄金系统氨基酸分析仪,在锂柱生理体液分析方法基础上,建立血清游离精氨酸(ARG)快速测定方法.血清样本经磺基水杨酸沉淀蛋白后取上清液进行色谱分析,色谱柱为Beckman公司阳离子交换柱(12cm ×4.0 mm);流动相为20 mmol*L-1柠檬酸锂水溶液,流速为20 ml*h-1;比色波长570nm.该法检测精氨酸浓度的线性范围为5mg*L-1~50 mg*L-1,相关系数0.99834,最低检测限1 mg*L-1,重复性:日内RSD 0.40%,日间RSD 0.55 % ,回收率97.67%~100.67%(平均值99.07%);整个实验过程耗时28min.该法简便、快速、准确、可靠,适用于临床和科研工作.
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