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Construction and Expression of the H Chain 6 × CDR3 Gene of Anti-idiotypic Monoclonal Antibody NP30 of Schistosoma japonicum/IL-2 Fusion Gene
Author(s): 
Pages: 299-301
Year: Issue:  4
Journal: ACTA UNIVERSITATIS MEDICINALIS NANJING

Keyword:  日本血吸虫融合基因抗体抗独特型白细胞介素-2;
Abstract: 目的:构建日本血吸虫单克隆抗独特型抗体NP30重链可变区6×CDR3基因/IL-2融合基因,以观察其对动物的抗血吸虫感染保护作用.方法:通过PCR方法体外扩增NP30 6×VHCDR3和IL-2基因,经测序后先后重组入原核表达质粒pET-28a(+)相应的位点上,将构建正确的6×VHCDR3/IL-2-pET-28a(+)表达质粒转化E coli BL21,IPTG诱导表达.结果:构建的6×VHCDR3/IL-2融合基因中,6×VHCDR3和IL-2序列均正确,融合基因经IPTG诱导表达重组蛋白,十二烷基磺酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)分析分子质量约为30 ku,表达量约占菌体总蛋白的20%.结论:成功构建并原核表达了NP30 6×VHCDR3/IL-2融合基因.
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