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The Expression of 8-oxo-dGTPase in Human Lymphocytes by Oxidative Stress
Author(s): 
Pages: 328-330
Year: Issue:  4
Journal: ACTA UNIVERSITATIS MEDICINALIS NANJING

Keyword:  氧化应激淋巴细胞8-羟基-2'-脱氧鸟苷三磷酸酶;
Abstract: 目的:探讨人外周血淋巴细胞遭受H2O2损伤时,8-羟基-2'-脱氧鸟苷三磷酸酶(8-oxo-dGTPase)表达的变化.方法:体外培养外周血淋巴细胞,然后分为正常对照组、氧化组和抗氧化组,采用逆转录PCR及凝胶成像技术,测定8-oxo-dGTPase的mRNA表达水平.结果:人淋巴细胞经H2O2持续作用,其8-oxo-dGTPase表达的相对水平也明显增加;而经维生素C抗氧化处理后,8-oxo-dGTPase表达的水平与正常对照组相比则无明显改变.结论:随着氧化应激的持续作用,细胞8-oxo-dGTPase的表达也明显增加,提示8-oxo-dGTPase可以作为氧化应激的标记物;而预先给予维生素C处理,可以明显抑制H2O2所造成的DNA损伤.
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