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Dynamic distribution of histone H3 Ser10 phosphorylation during meiosis in spermatocytes of the silkworm, Bombyx mori
Author(s): 
Pages: 302-308
Year: Issue:  3
Journal: Acta Entomologica Sinica

Keyword:  Bombyx moriH3 Ser10 phosphorylationmeiosisenpyrene spermatozoonapyrene spermatozooncell cycle;
Abstract: 【Aim】 To explore the function of histone H3 Ser10 phosphorylation(H3 Ser10 ph) during meiosis in spermatocytes of the silkworm, Bombyx mori. 【Methods】 The testis tissues of B. mori from the 4 th instar larval stage to pupal stage were dissected and separated. The slides of testis tissues at different stages of meiosis in spermatocytes were prepared by encapsulation of acrylamide gel and then the localization characteristics of the H3 Ser10 ph antibody was detected by using immunofluorescence staining. 【Results】 The phosphorylation of histone H3 Ser10 occurred in the specific position of pachytene chromosomes during meiosis in eupyrene spermatocytes of B. mori. The signal of H3 Ser10 ph was gradually weakened in the diplotene, and no phosphorylation signal was detected in the chromosomes at the diakinesis. With the progress of cell cycle, phosphorylation signal began to increase, and reached the highest level at the metaphase of the first meiosis. When the cells entered the prometaphase Ⅱ of meiosis, the signal of H3 Ser10 ph on the chromosome arm disappeared, and there was holo-shaped fluorescent signal of the H3 Ser10 ph antibody close to the spindle microtubulin attaching side. At the end of meiosis Ⅱ, a weak H3 Ser10 ph signal remained in the specific position of chromatin. During apyrene spermatocyte meiosis, the signal of H3 Ser10 ph was aggregated uniformly along the whole chromosomes from metaphase Ⅰ to telophase Ⅰ, and the spindle microtubules were parallel to the equatorial plane at anaphase Ⅰ. 【Conclusion】 Histone H3 Ser10 phosphorylation is correlated with the dynamic changes of chromatin in eupyrene spermatocytes and apyrene spermatocytes of the silkworm.
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