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Expression of Cry1Ab/Cry1Ac fusion protein in the transgenic cry1Ab/cry1Ac maize and its control efficacy against the Asian corn borer, Ostrinia furnacalis (Lepidoptera: Crambidae), in the laboratory
Author(s): 
Pages: 1201-1206
Year: Issue:  10
Journal: Acta Entomologica Sinica

Keyword:  Ostrinia furnacalistransgenic cry1Ab/cry1Ac maizecontrol efficacyprotein expression levellaboratory bioassay;
Abstract: 【Aim】 Evaluating the efficacy of transgenic Bt maize for resistance to target insect in the laboratory is a key part of the research and development and the environmental safety assessment of insect resistant transgenic maize. 【Methods】 The expression levels of Cry1 Ab/Cry1 Ac fusion protein in fresh whorl leaves of transgenic cry1 Ab/cry1 Ac maize ZZM030 were quantified by using enzyme-linked immunosorbent assay(ELISA). The survival rates of neonates of the susceptible strain(ACB-BtS), Cry1 Ab-resistant strain(ACB-AbR) and Cry1 Ac-resistant strain(ACB-AcR) of the Asian corn borer(ACB), Ostrinia furnacalis, fed on whorl leaves of the transgenic maize ZZM030 and non-transgenic maize X249 were determined by using laboratory bioassays. 【Results】 The expression levels of Cry1 Ab/Cry1 Ac fusion protein in the whorl leaves of the transgenic maize ZZM030 at the 4-leaf stage and 8-leaf stage were 10.62 and 2.94 μg/g FW, respectively. The survival rates of ACB-BtS neonates fed on whorl leaves of the transgenic maize ZZM030 for 2 d and more than 4 d were only 23.6% and 0, respectively, while the survival rate of ACB-BtS neonates fed on the non-Bt control maize X249 for 4 d was 93.1%. The survival rates of ACB-AbR and ACB-AcR neonates fed on whorl leaves of the transgenic maize ZZM030 for 6 d were 11.1% and 12.5%, respectively, whereas those fed on whorl leaves of non-Bt maize X249 for 6 d were 81.9% and 77.8%, respectively. 【Conclusion】 The highly expressed Cry1 Ab/Cry1 Ac fusion protein in transgenic cry1 Ab/cry1 Ac maize ZZM030 has an excellent control efficacy against ACB neonates.
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