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da shu tai xin xi bao de fen li pei yang ji shu
Author(s): 
Pages: 235-237
Year: Issue:  3
Journal: THE JOURNAL OF PRACTICAL MEDICINE

Keyword:  心肌细胞培养;
Abstract: 目的:介绍一套高效率的大鼠胎心细胞的分离培养方法及其应注意的事项.方法:无菌条件下取胎心,经酶解消化法分离胎心细胞,通过差速贴壁和添加Brdu来提高胎心细胞纯度.结果:培养的心肌细胞的纯度可达94%,活细胞数>85%,形态典型,可同步自律搏动达2~3周.结论:该方法可为实验研究提供纯度高、活细胞数较多、状态良好的胎心细胞.
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