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Method of differentiation of adult humen bone marrow mesenchymal stem cell into Schwann-like cells in vitro
Author(s): 
Pages: 202-206
Year: Issue:  2
Journal: JOURNAL OF PEKING UNIVERSITY(HEALTH SCIENCES)

Keyword:  间充质干细胞骨髓细胞分化雪旺样细胞;
Abstract: 目的:建立人骨髓间充质干细胞体外向雪旺样细胞定向诱导分化的方法.方法:取健康人骨髓血标本,利用percoll(密度为1.073 g*ml-1)分离骨髓单个核细胞,在DMEM-LG+10%(体积分数)FBS中培养,通过流式细胞术对培养细胞进行表型测定,对第2、3、5、8代间充质干细胞进行定向诱导分化,用单抗S-100、神经胶质纤维酸性蛋白(glial fibrillarg acidic protein,GFAP),通过免疫组化方法对诱导的细胞进行鉴定.结果:经percoll分离的间充质干细胞可传16代,细胞数增加了大约6×107倍.流式细胞术结果显示:percoll分离出来的未经培养的细胞,其CD29+CD44+CD34-CD45-细胞数为32.47%±3.49%,而培养后贴壁细胞中CD29+CD44+CD34-CD45-细胞数为94.38%±1.50%.诱导后免疫组化染色结果显示:由β-ME +bFGF预诱导、β-ME +bFGF诱导的S-100阳性细胞最多,可达90%±4%,GFAP阳性细胞为21%±5%.结论:人骨髓中间充质干细胞能定向诱导分化成雪旺样细胞.
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