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DNA Samples Preparation from Single Cell and its Application in Sensitivity Test
Author(s): 
Pages: 6-8
Year: Issue:  1
Journal: JOURNAL OF FORENSIC MEDICINE

Keyword:  灵敏度分析DNA样本制备单个细胞;
Abstract: 目的建立一种用于高精确灵敏度分析的微量DNA样本制备方法.方法用显微捕获技术制备以细胞为单位的DNA样本,并用于Proifiler Plus试剂盒及ABI 310遗传分析仪的灵敏度测试.结果建立了单细胞捕获操作方案,成功制备了依次含1~11个细胞的一组DNA分析样本,并可准确用于Profiler Plus(R)试剂盒及ABI 310遗传分析仪的灵敏度研究.结论基于显微操作技术的高精确微量DNA分析样本制备方法可准确用于DNA分析技术方法的灵敏度研究.
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