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CLONING OF HUMAN TELOMERASE REVERSE TRANSCRIPTASE GENE EXON 16
Author(s): HAO Zhiming, LUO Jinyan, CHENG Jin, WANG Quanying, YANG Guangxiao
Pages: 1-
3
Year: 2003
Issue:
2
Journal: CHINA JOURNAL OF MODERN MEDICINE
Keyword: 人端粒酶逆转录酶; 外显子; PCR; 基因克隆;
Abstract: 目的:克隆人端粒酶逆转录酶(human telomerase reverse transcriptase hTERT)基因第16外显子,用于后续研究.方法:使用Pfu DNA聚合酶以PCR法从人外周血白细胞DNA中扩增出包括hTERT第16外显子的891bpDNA片段,连接于pGEM-T easy vector,酶切筛选重组克隆,测序证实序列的正确性.结果:PCR产物电泳证实有符合大小的条带,连接及转菌后酶切筛选出的重组克隆经测序证实序列正确性可达99.9%.结论:本研究成功克隆了hTERT第16外显子,可用于3'UTR对hTERT表达调节研究及以hTERT为靶的肿瘤基因治疗研究.
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