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Effects of edaravone on proinflammatory cytokines in BV-2 microglia activated by lipopolysaccharide
Author(s): YU Shu-bei, LIN Shu, Department of Pharmacy, Second Affiliated Hospital of Wenzhou Medical University
Pages: 1244-
1246
Year: 2019
Issue:
12
Journal: The Chinese Journal of Clinical Pharmacology
Keyword: edaravone; microglia; lipopolysaccharide;
Abstract: Objective To investigate the effect of edaravone on proinflammatory cytokines in BV-2 microglia activated by lipopolysaccharide(LPS).Methods BV-2 microglia cells were assigned to 3 groups:model group,test group and control group.The control group was treated with 0.9% NaCl,and the model group was treated with 1 μg·mL-1 LPS,the test group was pretreated with 100 μmol·L-1 edaravone on the basis of modeling.The contents of nitric oxide(NO) and tumor necrosis factor-α(TNF-α) in the supernatant of BV-2 cells was detected by nitrate reductase and enzyme-linked immunosorbent assay,and the expression of phosphoinositide-3-kinase(PI3K)/protein kinase B(Akt)/nuclear factor kappa B(NF-κB) signaling pathway protein were detected by Western blot.Results After 24 hours of treatment,the contents of NO in the supernatant of BV-2 cells in control group,model group and test group were(9.13 ± 1.36) μmol·L-1,(16.89 ± 1.68)μmol·L-1,(11.58 ± 1.75) μmol·L-1,respectively; the contents of TNF-α in the supernatant of BV-2 cells were(0.36 ± 0.11)pg·mL-1,(2569.54 ± 126.48) pg·mL-1,(1689.47 ± 102.58) pg·mL-1,respectively; the relative expression of p-PI3K protein was 0.58 ± 0.12,1.33 ± 0.21,0.52 ± 0.06,respectively; the relative expression of p-PI3K protein was 0.23 ± 0.06,0.78 ± 0.11,0.52 ± 0.14,respectively; the relative expression of p-IκB protein was 0.36 ± 0.05,1.36 ± 0.21,0.32 ± 0.04,respectively.There were statistically significant differences between the model group and the control/test group(P < 0.05).Conclusion Edaravone can regulate the activation of BV-2 cells and inhibit the expression of proinflammatory cytokines,which may be related to the inhibition of PI3K/Akt/NF-κB signaling pathway.
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