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Issue:
Tissue Culture Technique with the Shoot Tip of Zingiber of ficinale
Author(s):
HUANG Yuan-xin
,
WANG Ji-chun
,
TANG Dao-bin
,
HE Feng-fa
Pages:
24
-
25
Year:
2005
Issue:
1
Journal:
GUIZHOU AGRICULTURAL SCIENCES
Keyword:
生姜
;
茎尖
;
组织培养
;
移栽
;
Abstract:
用不同生姜品种竹姜与黄姜幼嫩根茎经高温预培后,剥取茎尖分生组织在MS+1.0mg/L 6-BA+0.5~1.0mg/LNAA培养基上进行离体培养,30~40d后分化出1.9个小芽,此时将细胞分裂素6-BA浓度增加到2.0~3.0mg/L,培养30d小芽增殖4.2个以上.生长素NAA是培育壮苗的重要激素,移栽苗成活率达90%以上.
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