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Resistance gene for plasmid pBL29 of B.licheniformis 29
Author(s): 
Pages: 36-39
Year: Issue:  1
Journal: JOURNAL OF HENAN UNIVERSITY OF SCIENCE & TECHNOLOGY(AGRICULTURAL SCIENCE)

Keyword:  质粒pBL29卡那霉素抗性因子原生质体基因转移;
Abstract: 采用改良碱裂解法对30株益生芽孢杆菌进行质粒抽提,仅从1株地衣芽孢杆菌B.licheniformis29菌株中抽提得到质粒pBL29,纯化后通过琼脂糖凝胶电泳观察,确证质粒pBL29为闭合环状、分子量3.7kb左右.通过药敏试验、吖啶橙消除试验、十二烷基磺酸钠消除试验及原生质体转化试验证明:B.licheniformis29菌株具有卡那霉素抗性,而且卡那霉素抗性基因是质粒编码而不是染色体编码.
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