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Expression of Glycoprotein B from Marek's Disease Virus in Recombinant Fowlpox Virus
Author(s): DING Qiaoling, CHEN Zuoyan
Pages: 64-
66
Year: 2003
Issue:
1
Journal: ACTA VETERINARIA ET ZOOTECHNICA SINICA
Keyword: 马立克氏病病毒; 糖蛋白B抗原; 重组鸡痘病毒;
Abstract: 将马立克氏病病毒gB基因用EcoR Ⅰ从质粒pUR-MDVEgB中切下.两端补平后,插入到质粒pEFgpt12s的Pst Ⅰ酶切位点.构建了含有完整的MDVgB基因的转移载体pEFgpt12s-gB.这个载体的特点是,它含有两个启动子,在强的复合启动子Spromoter的下游是插入的gB基因,另一个反向启动子vvP7.5的下游是标记基因Ecogpt,它的两端是FPV的非必需区.携带gB基因的质粒pEFgpt12s-gB通过磷酸钙的方法转染用282E4株FPV感染3-4 h的鸡胚成纤维细胞.通过药物筛选,得到含有gB基因的重组病毒.通过PCR、免疫荧光检测,证实了重组病毒中含有gB基因,并且gB糖蛋白也得到了表达.
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