Salivary microbial communities associated with severe early childhood caries
Author(s): Sun Tongzheng, Teng Fei, Jia Songbo, Tang Yongping, Jiang Ming, Huang Shi, Yuan Xiao, Li Xiaolan, Yang Fang
Pages: 150-155
Year: 2018 Issue: 2
Journal: West China Journal of Stomatology
Keyword: early childhood caries; oral microbiota; saliva; metagenomic;
Abstract: Objective To compare the salivary microbial profiles of healthy subjects and those with severe early childhood caries (S-ECC) by using high-throughput sequencing. Methods Salivary samples were obtained from children with S-ECC (group C, n=24) and healthy children (group H, n=24). Total metagenomic DNA was extracted, and DNA amplicons of the V1-V3 hypervariable region of the 16S rRNA gene were generated and subjected to 454 sequencing. The characteristics of oral microbial communities from the two groups were compared based on microbial diversity and taxonomy assignment. Results First, the microbial richness was significantly higher in group C than group H (P<0.05). Second, the microbial com-munity structure was significantly different for the groups H and C (P<0.01). In addition, caries microbiota was significantly conserved in group C (P<0.001). High expression of suspected cariogenic microorganisms in group C (P<0.1) and health related microorganisms in group H (P<0.1) were identified. Finally, models of caries risk assessment were proposed to distin-guish caries from healthy subjects with over 70% accuracy. Conclusion Salivary microbiota and certain taxa, such as caries-associated taxa(Prevotella),may be useful to screen/assess the children's risk of developing caries.
Pages: 150-155
Year: 2018 Issue: 2
Journal: West China Journal of Stomatology
Keyword: early childhood caries; oral microbiota; saliva; metagenomic;
Abstract: Objective To compare the salivary microbial profiles of healthy subjects and those with severe early childhood caries (S-ECC) by using high-throughput sequencing. Methods Salivary samples were obtained from children with S-ECC (group C, n=24) and healthy children (group H, n=24). Total metagenomic DNA was extracted, and DNA amplicons of the V1-V3 hypervariable region of the 16S rRNA gene were generated and subjected to 454 sequencing. The characteristics of oral microbial communities from the two groups were compared based on microbial diversity and taxonomy assignment. Results First, the microbial richness was significantly higher in group C than group H (P<0.05). Second, the microbial com-munity structure was significantly different for the groups H and C (P<0.01). In addition, caries microbiota was significantly conserved in group C (P<0.001). High expression of suspected cariogenic microorganisms in group C (P<0.1) and health related microorganisms in group H (P<0.1) were identified. Finally, models of caries risk assessment were proposed to distin-guish caries from healthy subjects with over 70% accuracy. Conclusion Salivary microbiota and certain taxa, such as caries-associated taxa(Prevotella),may be useful to screen/assess the children's risk of developing caries.
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