Author(s): Lei Wei, Ma Yue, Ren Aishu
Pages: 23-28
Year: 2018 Issue:  1
Journal: West China Journal of Stomatology
Keyword:  neurotrophin 3; human dental follicle cells; osteogenic differentiationn;
Abstract: Objective This study aims to investigate the effect ofneurotrophin 3 (NT-3) on the osteogenic differentiation of human dental follicle cells (hDFCs).Methods hDFCs were isolated and cultured in vitro.Immunocytochemical staining was used to identify the origin ofhDFCs.The effects of different NT-3 concentrations on hDFCs proliferation were detected by using CCK-8 assay.The alkaline phosphatase (ALP) activities and mRNA expression levels of bone morphogenetic protein-2 (BMP-2) and osteocalcin (OCN) were determined to investigate the effects of NT-3 on hDFCs osteogenesis.The difference in the number of mineralized nodules was detected using alizarin red staining.Results Vimentin and cytokeratin staining results showed that hDFCs originated from the mesenchymal cells.NT-3 exerted no evident effect on hDFCs proliferation.The ALP activity and the BMP-2 and OCN rnRNA expression levels of hDFCs were significantly improved under treatment with different NT-3 concentrations (25,50,and 100 ng·mL-l) compared with those in the control group.BMP-2 and OCN mRNA relative expression levels of hDFCs reached the highest when the NT-3 concentration was 100 ng·mL-1.The number of mineralized nodules reached the maximum when the hDFCs were treated with 50 and 100 ng·mL-1 NT-3.Conclusion Appropriate mass concentration of NT-3 can promote the osteogenic differentiation of hDFCs.