The server is under maintenance between 08:00 to 12:00 (GMT+08:00), and please visit later.
We apologize for any inconvenience caused
Login  | Sign Up  |  Oriprobe Inc. Feed
China/Asia On Demand
Journal Articles
Laws/Policies/Regulations
Companies/Products
Effect and Mechanism of Propranolol on Proliferation,Migration,and Angiogenesis of Human Melanoma A375 cells
Author(s): 
Pages: 139-143
Year: Issue:  2
Journal: Journal of Oral Science Research

Keyword:  PropranololMelanomaVEGFMMP-9CXCR4;
Abstract: Objective:To investigate the effects and its mechanism of different dose of propranolol on the proliferation,migration,and angiogenesis of human melanoma A375 cells.Methods:A375 cells were treated with 1,5,10 and 20μmol/L propranolol respectively in vitro.MTT method was used to detect the effects of propranolol on the proliferation ability of A375 cells.Wound healing assay was used to detect the effect of propranolol on the cell migration.Tube formation assay was used to investigate the angiogenesis ability of A375 cells on the HUVEC.RTPCR and western bolt were used to assay the effect of propranolol on the expression of VEGF,MMP-9,and CXCR4.Results:Propranolol did not significant affect the proliferation of A375 cells.It might suppress the migration,tube formation,and pro-angiogenesis ability of A375 cells by inhibiting the expression of VEGF,MMP-9,and CXCR4 in a dose dependent manner.Conclusion:Propranolol may play a role in suppressing the migration and angiogenesis of A375 cells by inhibiting the expression of VEGF,MMP-9,and CXCR4.
Related Articles
loading...