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Effect and Mechanism of Propranolol on Proliferation,Migration,and Angiogenesis of Human Melanoma A375 cells
Author(s): ZOU Hai-xiao, YANG Hai-li, ZHAO Yi-fang, SONG Li, Second Affiliated Hospital of Nanchang University, The State Key Laboratory Breeding Base of Basic Science of Stomatology, Hubei Province & Key Laboratory of Oral Biomedicine, Department of Oral and Maxillofacial Head And Neck Cancer Surgery, Stomatology Hospital of Wuhan University
Pages: 139-
143
Year: 2018
Issue:
2
Journal: Journal of Oral Science Research
Keyword: Propranolol; Melanoma; VEGF; MMP-9; CXCR4;
Abstract: Objective:To investigate the effects and its mechanism of different dose of propranolol on the proliferation,migration,and angiogenesis of human melanoma A375 cells.Methods:A375 cells were treated with 1,5,10 and 20μmol/L propranolol respectively in vitro.MTT method was used to detect the effects of propranolol on the proliferation ability of A375 cells.Wound healing assay was used to detect the effect of propranolol on the cell migration.Tube formation assay was used to investigate the angiogenesis ability of A375 cells on the HUVEC.RTPCR and western bolt were used to assay the effect of propranolol on the expression of VEGF,MMP-9,and CXCR4.Results:Propranolol did not significant affect the proliferation of A375 cells.It might suppress the migration,tube formation,and pro-angiogenesis ability of A375 cells by inhibiting the expression of VEGF,MMP-9,and CXCR4 in a dose dependent manner.Conclusion:Propranolol may play a role in suppressing the migration and angiogenesis of A375 cells by inhibiting the expression of VEGF,MMP-9,and CXCR4.
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