Author(s): ZHANG Ying, CHEN Hong-mei, WU Xinyi
Pages: 74-78
Year: 2017 Issue:  6
Journal: Journal of Otolaryngology and Ophthalmology of Shandong University
Keyword:  Diabetes mellitus; Cornea; Wound healing; Insulin-like growth factor-1;
Abstract: Objective To explore the expression of IGF-1 and IGF-1R in corneal wound healing in diabetic rats. Method Male Wistar rats were injected with streptozocin;serum glucose levels and body weight were monitored. Fol-lowing corneal abrasion, healing rate was evaluated. Real-time PCR and immunofluorescence were used to explore the expression of IGF-1 and IGF-1R. Result Low weights and high glucose levels were observed in diabetic rats. Healing rate was significantly lower in the diabetic rats than in normal rats. mRNA and protein expression of IGF-1 and IGF-1R was significantly lower in the diabetic rat group than in the normal group. Conclusion In diabetic rats, delayed corneal epithelial wound healing might be associated with impaired IGF-1 and IGF-1R expression.