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Study on Identification of special marker in DNA molecule of High royal jelly bee Api mellifera
Author(s): 
Pages: 3-4
Year: Issue:  8
Journal: JOURNAL OF BEE

Keyword:  意蜂高产浆聚合酶链反应(PCR)随机引物特异标记;
Abstract: 第一部分分离提取三品系意蜂(浙农大、萧山、平湖)的DNA,使用紫外分光光度计,波长260nm鉴定DNA纯度;第二部分以W(′-CGGCCCCGGT-3′)为随机引物进行PCR,扩增,电泳,获得DNA多态性图谱;第三部分比较三品系意蜂工蜂与雄蜂的DNA多态性图谱。结果凡是三品系意蜂的工蜂均出现一高产浆特异标记——W316pb条带,但三品系意蜂的雄蜂均无W316bp条带。则进一步鉴定了W316bp是高产浆意工蜂DNA分子的特异标记。
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