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Screening and characterization of proteins from entomogenous fungi toxic to Monochamus alternatus ( Coleoptera : Cerambycidae)
Author(s): 
Pages: 1-6
Year: Issue:  1
Journal: ACTA ENTOMOLOGICA SINICA

Keyword:  虫生真菌松墨天牛毒蛋白筛选分子量等电点;
Abstract: 从6株虫生真菌中分离纯化了48种蛋白.以松墨天牛Monochamusalternatus为试虫,通过生测筛选出4种高毒性蛋白,并从4种高毒性蛋白中筛选出毒力最强的蛋白Bb36W-D.蛋白Bb36W-D含2个亚基,大亚基和小亚基的分子量分别约为24kD和17 kD;等电点分别为9.47和9.32.该蛋白含16种氨基酸,其中精氨酸、组氨酸及丙氨酸含量较高.
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