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Optimization of the gel-filtration chromatography parameters for separating proteins from the crude extracts of housefly
Author(s): 
Pages: 139-142
Year: Issue:  1
Journal: ACTA ENTOMOLOGICA SINICA

Keyword:  家蝇蛋白粗提液凝胶过滤层析分离;
Abstract: 考察了凝胶过滤介质、层析柱直径、柱床高度和洗脱流速对家蝇Musca domestica蛋白粗提液分离效果的影响,结果表明:凝胶过滤介质种类、层析柱直径、柱床高度、洗脱流速均能不同程度地影响家蝇蛋白粗提液的分离效果.在实验范围内,选择1.3 cm直径、40cm柱床高度的Sephadex G-75,以0.4 mL/min的流速洗脱时,对家蝇蛋白粗提液的分离效果比较理想.
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