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The Optimization of Reproducible Medium for Bifidobacterium adolescent
Author(s): 
Pages: 15-19
Year: Issue:  7
Journal: FOOD AND FERMENTATION INDUSTRIES

Keyword:  青春双歧杆菌增菌培养基优化;
Abstract: 针对青春双歧杆菌的营养需要,采用正交实验优化基础培养基及增殖因子的用量.得出最佳基础培养基配比是:大豆蛋白胨1.2%,胰蛋白胨0.6%,酪蛋白水解物0.6%,牛肉浸膏0.6%,葡萄糖1%,低聚糖0.5%,乳糖0.5%,酵母浸出粉0.7%.最佳增殖因子添加量是:胡萝卜汁15%,番茄汁5%,肝浸液15%,胱氨酸0.05%.最后使用优化出的增菌培养基配比测定生长曲线,同时测定pH和滴定酸度的变化,确定增茵培养终止时间为12 h,通过平板菌落计数,此时茵数可达2.9×109 cfu/mL.
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