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Fat content measurement of animal livers using quantitative computed tomography
Pages: 466-470
Year: Issue:  5
Journal: Radiologic Practice

Abstract: Objective:To study the fat content measurement of animal livers in vitro by quantitative computed tomography (QCT) using chemical Soxhlet extraction technique as the standard reference.Methods:Five pig livers,5 goose livers and 10 duck livers were collected and QCT as well as chemical Soxhlet extraction were used individually to measure fat content for each sample.Fat percentage of all samples determined by QCT (Fat%QCT) and by Soxhlet chemical extraction (Fat%Soxhlet) was compared.Furthermore,linear regression analysis was used to detect the relationship between Fat%QCT and Fat%Soxhlet.Results:The fat percentage of pig liver,duck liver and goose liver measured by chemical extraction ranged from 0.5% to 2.3%,7.2% to 21%,and 35% to 52.6% respectively.The fat content of liver measured by QCT was lower than that measured by chemical Soxhlet extraction method,but there was no statistical significant difference between results of two methods (P =0.052).The two measurements were highly correlated (r =0.962,P < 0.001).Fat % Soxhlet could be predicted by Fat % QCT with the equation as:Fat % Soxhlet =0.74 Fat % QCT+ 7.272 (r2 =0.965,P < 0.001).Conclusion:Fat content of animal liver can be measured accurately with a conventional computed tomography (CT) scanner with QCT phantom and software compared to the chemical Soxhlet extraction as standard reference.
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