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Effects of Simulated Weightlessness with Random Positioning Machine on Microtubule Actin Crosslinking Factor 1 Expression and β-catenin Pathway in Osteoblast
Author(s): 
Pages: 7-11
Year: Issue:  1
Journal: Space Medicine & Medical Engineering

Keyword:  simulated weightlessnessMACF1osteoblastrandom positioning machine;
Abstract: 目的 研究随机回转(RPM)模拟失重条件对微管微丝交联因子1(MACF1)表达及β-catenin信号通路的影响,为失重性骨丢失机制研究提供实验依据.方法 RPM条件培养成骨细胞,PCR检测MACF1mRNA表达变化,Western blot检测MACF1以及不同磷酸化位点β-catenin蛋白表达变化,荧光素酶报告基因检测细胞β-catenin荧光素酶活性变化.结果 RPM模拟失重条件下,成骨细胞MACF1表达下降,β-catenin mRNA和不同位点磷酸化蛋白表达量发生改变;MACF1 shRNA抑制了成骨细胞中β-catenin表达,影响了β-catenin蛋白稳定性及活性.结论 RPM模拟失重抑制了MACF1表达和β-catenin表达及活性;MACF1 shRNA影响了β-catenin表达及活性.MACF1可能是一种力敏感分子,通过调控β-catenin拳与成骨细胞对力学信号的感知和响应.
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