The server is under maintenance between 08:00 to 12:00 (GMT+08:00), and please visit later.
We apologize for any inconvenience caused
Login  | Sign Up  |  Oriprobe Inc. Feed
China/Asia On Demand
Journal Articles
Laws/Policies/Regulations
Companies/Products
Bookmark and Share
Identification and characterization of peroxiredoxin 2 involved in the antioxidative response induced by bacterial infection in the pea aphid,Acyrthosiphon pisum (Hemiptera:Aphididae)
Author(s): 
Pages: 254-263
Year: Issue:  3
Journal: Acta Entomologica Sinica

Keyword:  Acyrthosiphon pisumperoxiredoxinreactive oxygen species(ROS)antioxidative responsebacterial infection;
Abstract: 【Aim】The aim of this study is to reveal the function of peroxiredoxin 2(Ap Prx2)in the pea aphid,Acyrthosiphon pisum during bacterial infection.【Methods】The open reading frame of ApP rx2 was cloned and heterologously expressed in Escherichia coli.The antioxidative function of the purified recombinant Ap Prx2 was characterized.The H2O2concentration and ApP rx2 transcription level were detected in pea aphids after bacterial infections of Pseudomonas aeruginosa and Staphylococcus aureus.RNA interference was used to knockdown the expression of ApP rx2.H2O2concentration,bacterial load and the survival rate of aphids were evaluated after the knockdown of ApP rx2.【Results】Sequence alignment showed that Ap Prx2 is highly similar to 2-Cys Prxs from other insect species.In vitro assays demonstrated that the recombinant Ap Prx2 protein degraded H2O2,and ApP rx2 expression rendered E.coli cells resistant to H2O2.Infections of the bacteria P.aeruginosa and S.aureus increased the H2O2concentration and ApP rx2 expression in the pea aphids.The knockdown of ApP rx2expression increased the H2O2concentration,reduced the bacteria load and increased the mortality rate of aphids infected with S.aureus.【Conclusion】Ap Prx2 functions as an antioxidative protein protecting pea aphids from stress induced by infection with bacteria,particularly S.aureus.
Related Articles
No related articles found