Author(s): Gu Xin, Shang Jun, Zhang Wen-gang, Jiang Qin
Pages: 225-229
Year: 2017 Issue:  3
Journal: Chinese Journal of Antibiotics
Keyword:  Enterococcus faecalis; Antimicrobial resistance; Genotype; Antimicrobial agents;
Abstract: Objective To investigate the antibiotic susceptibility and resistance genotypes in Enterococcus faecalis strains isolated from ten large-scale pig farms in Shanghai.Methods 133 strains of Enterococcusfaecalis were collected from March 2013 to May 2014.The susceptibility testing was performed by broth microdilution,and the genotypes of [β-lactam antibiotics resistance-associated gene(TEM),aminoglycoside antibiotics resistanceassociated genes(aac(6')/aph(2"),aph(3')-Ⅲ and ant(6)-Ⅰ),tetracycline resistance-associated gene(tetM),erythromycin resistance-associated genes(ermB and mefA) and vancomycin resistant-related genes(vanA and vanB) were analyzed by PCR.Results The positive rate of the resistance genes ermB,tetM,TEM,ant(6)-Ⅰ,aac(6')/ aph2",aph(3')-Ⅲ and vanA in the 133 strains of Enterococcusfaecalis tested were 95.5％(127/133),91.0％(121/133),63.2％(84/133),45.9％(61/133),42.1％(56/133),24.1％(32/133) and 3.0％(4/133),respectively.The majority (81.2％)displayed multidrug resistance with 6 to 9 resistance as the main resistant strains was serious in the susceptibility testing against 12 antimicrobial agents of the 133 strains of Enterococcusfaecalis.Conclusion This study shows that multidrug resistance of Enterococcusfaecalis was a serious issue,and harboring resistance genes against antimicrobial agents was an important reason for the resistance to antibiotics in Enterococcusfaecalis.