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Monitoring and mechanisms of imidacloprid resistance in Aphis gossypii( Hemiptera: Aphidiidae) in the main cotton production areas of China
Pages: 1246-1253
Year: Issue:  11
Journal: Acta Entomologica Sinica

Keyword:  Aphis gossypiiimidaclopridresistanceresistance monitoringenzyme activityn AChR β1 subunit;
Abstract: 【Aim】Aphis gossypii is easy to produce resistance to insecticides because of its short growth cycle and high reproduction rate. This study aims to clarify the levels and mechanisms of imidacloprid resistance in A. gossypii in the main cotton production areas of China. 【Methods】Different populations of A. gossypii were collected from fields in Haidian of Beijing( BJ),Langfang( LF) and Handan( HD)of Hebei,Dezhou( DZ) of Shandong,Xuchang( XC) of Henan,and Kuitun( KT) and Akesu( AKS)of Xinjiang. Leaf-dipping method was used to determine the resistance levels of A. gossypii to imidacloprid. The activities of three detoxification enzymes [mixed function oxidase( MFO),carboxylesterase( Car E) and glutathione S-transferase( GST) ]and acetylcholinesterase( Ach E) in different A. gossypii populations were assayed. And the mutation of target gene( n AChR β1 subunit gene) was also detected.【Results】The BJ,XC and HD populations of A. gossypii were still susceptible to imidacloprid,while the LF,AKS,DZ and KT populations exhibited moderate levels of resistance to imidacloprid,with the resistance ratios of 22. 6-,26. 3-,53. 5-and 61. 1-fold,respectively. The activities of MFO,Car E and GST in the AKS and KT populations were significantly higher than those in the BJ susceptible population.The activities of MFO,Car E and GST in the AKS population were 1. 7-,1. 6-and 1. 5-fold,and those in the KT population were 1. 8-,1. 7-and 1. 5-fold as high as those in the BJ population,respectively. But there was no significant difference in acetylcholinesterase( Ach E) activities among the AKS,KT and BJ populations. A single point mutation in the n AChR β1 subunit causing an arginine to threonine substitution( R81T) observed in LF,AKS,DZ and KT populations was associated with the imidacloprid resistance in A. gossypii. 【Conclusion】The results suggest that enhancement of MFO,Car E and GST activities and the mutation( R81T) of n AChR β1 subunit might confer resistance to imidacloprid in A. gossypii.
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