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Abamectin resistance and expression of resistance-related genes in field populations of Tetranychus urticae( Acari: Tetranychidae) in China
Author(s): 
Pages: 1199-1205
Year: Issue:  11
Journal: Acta Entomologica Sinica

Keyword:  Tetranychus urticaeabamectininsecticide resistanceresistance-related genegene expression;
Abstract: 【Aim】The two-spotted spider mite,Tetranychus urticae Koch is an important pest on many agricultural crops worldwide. This study aims to understand the current abamectin resistance and the mutation frequency and expression change of resistance-related genes in different field populations of T.urticae in China. 【Methods】The resistance of eight field populations of T. urticae to abamectin was determined by vial-leaf dipping( VLD) method,and the gene mutation frequency related to abamectin resistance was detected and the expression levels of genes likely related to abamectin resistance in highly resistant populations were determined by quantitative RT-PCR technique. 【Results】All the eight field populations of T. urticae tested developed resistance to abamectin. Extremely high resistance to abamectin was found in the populations from Miyun,Beijing( MY-BJ),Weifang,Shandong( WF-SD),Sanya,Hainan( SY-HN),and Changsha,Hunan( CS-HN),with the resistance ratios of 1 526. 75-,481. 00-,315. 25-,and 160. 75-fold,respectively. In the populations from Tongxian,Beijing( TX-BJ),Haidian,Beijing( HD-BJ),Yuncheng,Shanxi( YC-SX),and Tai ’an,Shandong( TA-SD),high resistance was observed with the resistance ratios ranging from 54. 38-to 136. 38-fold. The mutation frequency of the glutamate-gated chloride ion channel gene Glu Cl related to abamectin resistance differed in the eight field populations. The highest and lowest mutation frequencies were found in MY-BJ( 91. 7%) and YC-SX( 13. 3%) populations,respectively,while moderate mutation frequencies were found in WF-SD and SY-HN populations. There was a significantly positive correlation between resistance level and the mutation frequency of Glu Cl( P < 0. 05). The expression levels of Glu Cl and γ-aminobutyric acid receptor gene( GABAR) in the resistant populations decreased significantly as compared with those in the relatively susceptible strain. 【Conclusion】Abamectin resistance exists widely in field populations of T. urticae in China. Resistance-related genes with point mutation and the decreased expression are likely relevant to resistance development. Abamectin should be avoided to be applied in controlling T.urticae in fields.
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