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Identification of host interacting proteins of influenza virus PB2 by yeast two-hybrid system
Author(s): 
Pages: 15-19
Year: Issue:  1
Journal: Chinese Journal of Preventive Veterinary Medicine

Keyword:  influenza virusPB2 proteinyeast two-hybridco-immunoprecipitationconfocal;
Abstract: The PB2 protein of influenza virus acts on the initiation stage of viral RNA transcription by assisting the formation of viral mRNA primer. In addition, PB2 protein is involved in the mammalian host adaptation process and has an effect on the host range tropism of influenza virus. In this study, we used a yeast two-hybrid system to screen a cDNA library prepared from a mixture of four human cell lines (A549, Calu-3, THP-and U251) for host proteins that interact with the PB2 protein of 2009 H1N1 influenza virus. One specific interacting partners of PB2, four and a half LIM domains 2 (FHL2), was selected for further study. The interaction between PB2 and FHL2 was confirmed by performing co-immunoprecipitation and confocal microscopy experiments. We found that overexpression of FHL2 protein in 293 cells by transient transfection negatively regulated the replication of influenza virus. This study laid a foundation to further reveal the role of FHL2 in regulating influenza virus life cycle by interacting with PB2 protein.
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