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M2 protein of avian influenza virus inhibits the degradation of autophagosome
Pages: 5-9
Year: Issue:  1
Journal: Chinese Journal of Preventive Veterinary Medicine

Keyword:  autophagyavian influenza virusM2 protein;
Abstract: Autophagy, as a self-protective mechanism of cells, plays an important role in host cells to resist virus infection. To explore the effects of avian influenza virus (AIV) M2 protein on autophagy, the pGFP-LC3 and pCAF-M2 recombinant plasmids were constructed and transfected into 293T or Hela cells by cationic liposome method, and the cells transfected with only plasmid were as negative control group, then the autophagosomes and autophagic flux were detected by indirect immunofluorescence assay and western blot. The results showed that M2 protein of AIV was able to induce the accumulation of autophagosomes. Moreover, the results also indicated that M2 protein mediated the accumulation of autophagosomes by blocking lysosomal degradation of autophagosomes detected by the LC3-Ⅱ turnover, tandem mRFP-GFP-LC3 fluorescence microscopy, pEGFP-LC3 and LAMP1 colocalization observation. These data suggest that M2 protein plays an important role for autophagy in AIV infected cells and lays the foundation for further study on the role of autophagy in the regulation of influenza A virus replication.
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