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Genetic analysis of porcine reproductive and respiratory syndrome virus in a large-scale pig farm in China during 2014-2016
Pages: 10-14
Year: Issue:  1
Journal: Chinese Journal of Preventive Veterinary Medicine

Keyword:  porcine reproductive and respiratory syndrome virusepidemiology;
Abstract: To investigate the prevalence of porcine reproductive and respiratory syndrome virus (PRRSV) in a large-scale pig farm during 2014 to 2016, a total of 117 clinical samples were collected from the suspected PRRSV infected pigs for PRRSV detection and sequence analysis of the variations of the virus. The results showed that 57 of 117 the samples were positive for PRRSV. The sequencing analysis of PRRSV NSP2 and ORF5 genes revealed the 43 PRRSVs in the positive samples were the HP-PRRSV during 2014-2016 and shared 93.6% to 99.6% identity with HuN4 in the NSP2 gene. While, 14 PRRSVs in the positive samples from 2016 had the 111+1+19 amino acid deletions in the NSP2 genes, which had same deletions with the PRRSVNADC30 strain, but the homologous in nucleotide sequences were only 92.3%to 92.9%for NSP2 and 93.8%to 94.8%for ORF5 genes. However, 4 PRRSVs from the positive samples in 2016 with the HP-PRRSV typical characters of 1+29 amino acid deletions in the NSP2, were classified into the lineage 3 represented by the PRRSV GM2 strain in ORF5 gene with 96.7% to 97.0% identity, which emerged in the regions of Hong Kong, Taiwan and Guangdong in recent years. These results demonstrated that PRRSV had undergone a rapid genetic variation even in the same pig farm.
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