The server is under maintenance between 08:00 to 12:00 (GMT+08:00), and please visit later.
We apologize for any inconvenience caused
Login  | Sign Up  |  Oriprobe Inc. Feed
China/Asia On Demand
Journal Articles
Bookmark and Share
Isolation and identification of the epizootic hemorrhagic disease virus from cattle in Guangdong
Pages: 67-70
Year: Issue:  1
Journal: Chinese Journal of Preventive Veterinary Medicine

Keyword:  Epizootic haemorrhagic disease viruscattleserotypeisolationidentification;
Abstract: To investigate the prevalence of epizootic hemorrhagic disease virus (EHDV) among ruminants in Guangdong province, the cattle blood samples were collected and detected by RT-PCR, then the positive samples were inoculated into BHK-21 cells for the virus isolation, and a virus was isolated. In addition, VP7 gene fragment was amplified by RT-PCR and sequenced. The alignment of the VP7 sequence showed that the isolate was high similarity (98%-100%) with other EHDV reference strains available in GenBank. The virus titer was 10-6.5 TCID50 in BHK-21 cells, and unable to agglutinate chicken red blood cells. All the results indicated that the isolate was EHDV, named GD-N133. Furthermore, combined with virus neutralization test and the VP2 gene fragment analysis for genotyping the virus, the genotype of the isolate was identified as EHDV-1.
Related Articles
No related articles found