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Isolation and identification of the epizootic hemorrhagic disease virus from cattle in Guangdong
Author(s): 
Pages: 67-70
Year: Issue:  1
Journal: Chinese Journal of Preventive Veterinary Medicine

Keyword:  Epizootic haemorrhagic disease viruscattleserotypeisolationidentification;
Abstract: To investigate the prevalence of epizootic hemorrhagic disease virus (EHDV) among ruminants in Guangdong province, the cattle blood samples were collected and detected by RT-PCR, then the positive samples were inoculated into BHK-21 cells for the virus isolation, and a virus was isolated. In addition, VP7 gene fragment was amplified by RT-PCR and sequenced. The alignment of the VP7 sequence showed that the isolate was high similarity (98%-100%) with other EHDV reference strains available in GenBank. The virus titer was 10-6.5 TCID50 in BHK-21 cells, and unable to agglutinate chicken red blood cells. All the results indicated that the isolate was EHDV, named GD-N133. Furthermore, combined with virus neutralization test and the VP2 gene fragment analysis for genotyping the virus, the genotype of the isolate was identified as EHDV-1.
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