Propofol protects against blood-spinal cord barrier disruption induced by ischemia/reperfusion injury
Author(s): Li-jie Xie, Jin-xiu Huang, Jian Yang, Fen Yuan, Shuang-shuang Zhang, Qi-jing Yu, Ji Hu
Pages: 125-132
Year: 2017 Issue: 1
Journal: Neural Regeneration Research
Keyword: nerve regeneration; spinal cord ischemia reperfusion injury; blood-spinal cord barrier; propofol; matrix metalloprotease-9; nuclear factor-κB; tight junction proteins; neural regeneration;
Abstract: Propofol has been shown to exert neuroprotective effects on the injured spinal cord. However, the effect of propofol on the blood-spinal cord barrier (BSCB) after ischemia/reperfusion injury (IRI) is poorly understood. Therefore, we investigated whether propofol could maintain the integrity of the BSCB. Spinal cord IRI (SCIRI) was induced in rabbits by infrarenal aortic occlusion for 30 minutes. Propofol, 30 mg/kg, was intravenously infused 10 minutes before aortic clamping as well as at the onset of reperfusion. hTen, 48 hours later, we per-formed histological and mRNA/protein analyses of the spinal cord. Propofol decreased histological damage to the spinal cord, attenuated the reduction in BSCB permeability, downregulated the mRNA and protein expression levels of matrix metalloprotease-9 (MMP-9) and nuclear factor-κB (NF-κB), and upregulated the protein expression levels of occludin and claudin-5. Our ifndings suggest that propofol helps maintain BSCB integrity atfer SCIRI by reducing MMP-9 expression, by inhibiting the NF-κB signaling pathway, and by maintaining expression of tight junction proteins.
Pages: 125-132
Year: 2017 Issue: 1
Journal: Neural Regeneration Research
Keyword: nerve regeneration; spinal cord ischemia reperfusion injury; blood-spinal cord barrier; propofol; matrix metalloprotease-9; nuclear factor-κB; tight junction proteins; neural regeneration;
Abstract: Propofol has been shown to exert neuroprotective effects on the injured spinal cord. However, the effect of propofol on the blood-spinal cord barrier (BSCB) after ischemia/reperfusion injury (IRI) is poorly understood. Therefore, we investigated whether propofol could maintain the integrity of the BSCB. Spinal cord IRI (SCIRI) was induced in rabbits by infrarenal aortic occlusion for 30 minutes. Propofol, 30 mg/kg, was intravenously infused 10 minutes before aortic clamping as well as at the onset of reperfusion. hTen, 48 hours later, we per-formed histological and mRNA/protein analyses of the spinal cord. Propofol decreased histological damage to the spinal cord, attenuated the reduction in BSCB permeability, downregulated the mRNA and protein expression levels of matrix metalloprotease-9 (MMP-9) and nuclear factor-κB (NF-κB), and upregulated the protein expression levels of occludin and claudin-5. Our ifndings suggest that propofol helps maintain BSCB integrity atfer SCIRI by reducing MMP-9 expression, by inhibiting the NF-κB signaling pathway, and by maintaining expression of tight junction proteins.
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