The Methods for Determination of Losartan Potassium Content
Author(s): Sun Haoli, Zhang Yi, Qian Youxiang, Liu Yue, The Yangtze River Pharmaceutical Group of Jiangsu HAI-CI Biological Pharmaceutical Co., Ltd.
Pages: 157-159
Year: 2016 Issue: 21
Journal: Guangdong Chemical Industry
Keyword: Losartan potassium; HPLC; determination of content;
Abstract: Objective: To optimize the determination method of Losartan potassium. Methods: High-performance-liquid-chromatography(HPLC) on a chromatographic column of Waters XBridge C18(4.6 mm×250 mm, 5 μm) was used; 0.005 mol/L potassium dihydrogen phosphate(p H=7.0) was used as mobile phase A and acetonitrile was used as mobile phase B for gradient elution at column temperature of 25 ℃, flow-rate of 1.0 m L/min and wavelength at 254 nm. Results: The main component and the related substances were completely separated. The relationship between response and the concentration in the range of 125~500 g·m L-1 was good; the precision of RSD was 0.19 %, the repeatability of RSD was 0.19 % and the intermediate precision of RSD was 0.31 %. The samples and the reference solutions were stable in seven days, which the average recovery rate were 99.12 %(RSD=0.18 %), 99.82 %(RSD=0.16 %) and 99.79 %(RSD=0.13 %) relatively; the durability of method were good with the change of column temperature, velocity ect. : The method was specific, reliable, accurate and reliable.
Pages: 157-159
Year: 2016 Issue: 21
Journal: Guangdong Chemical Industry
Keyword: Losartan potassium; HPLC; determination of content;
Abstract: Objective: To optimize the determination method of Losartan potassium. Methods: High-performance-liquid-chromatography(HPLC) on a chromatographic column of Waters XBridge C18(4.6 mm×250 mm, 5 μm) was used; 0.005 mol/L potassium dihydrogen phosphate(p H=7.0) was used as mobile phase A and acetonitrile was used as mobile phase B for gradient elution at column temperature of 25 ℃, flow-rate of 1.0 m L/min and wavelength at 254 nm. Results: The main component and the related substances were completely separated. The relationship between response and the concentration in the range of 125~500 g·m L-1 was good; the precision of RSD was 0.19 %, the repeatability of RSD was 0.19 % and the intermediate precision of RSD was 0.31 %. The samples and the reference solutions were stable in seven days, which the average recovery rate were 99.12 %(RSD=0.18 %), 99.82 %(RSD=0.16 %) and 99.79 %(RSD=0.13 %) relatively; the durability of method were good with the change of column temperature, velocity ect. : The method was specific, reliable, accurate and reliable.
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