PNIPAM hydrogel induces skeletal muscle inflammation response
Author(s): ZHU Rong, ZHANG Renfei, SHI Dandan, GU Ruicai, XIAO Jiangwei, LIU Xinghui, CAO Biao, LIAO Hua, Department of Anatomy, Key Laboratory of Construction and Detection of Guangdong Province, Southern Medical University, Clinical Laboratory, Third People’s Hospital of Mianyang City
Pages: 935-940
Year: 2016 Issue: 11
Journal: Immunological Journal
Keyword: PNIPAM hydrogel; Skeletal muscle; Inflammation; Mouse;
Abstract: In this study,we attempt to explore the possibility of the intramuscular implantation of the specific hydrogel to induce a chronic inflammatory-myoinjury model in mice.We injected temperature-sensitive PNIPAM hydrogel mixed with or without fast-type skeletal C protein into TA muscle of WT B6 mice,and analyzed mononuclear cell infiltration,muscle autoantigens expression,and the intramuscular CD8~+ T cells infiltration and MHC-I expression on the surface of new myofiber in TA muscle on day 15,30 and 60 days post-injection.Data showed that PNIPAM hydrogel could induce a skeletal muscle inflammation during 2 months.The inflammation response in hydrogel injecting area reached a peak on day 30,and then gradually decreased.Interestingly,the mixture of PNIPAM hydrogel/C-protein initiated a more severe and persistent muscle inflammatory response,than hydrogel injection alone.Immunofluorecence analysis demonstrated that the hydrogel induced the chronic muscle inflammation featured by the CD8~+ T cell infiltration and the increased class I MHC-positive regenerating myofibers.In conclusion,our work holds a new promise for preparing animal model of muscle inflammation using hydrogel as a bio-driver.And hydrogel-induced myositis model maybe useful for investigating pathologic mechanisms of muscle injury,inflammation and regeneration.
Pages: 935-940
Year: 2016 Issue: 11
Journal: Immunological Journal
Keyword: PNIPAM hydrogel; Skeletal muscle; Inflammation; Mouse;
Abstract: In this study,we attempt to explore the possibility of the intramuscular implantation of the specific hydrogel to induce a chronic inflammatory-myoinjury model in mice.We injected temperature-sensitive PNIPAM hydrogel mixed with or without fast-type skeletal C protein into TA muscle of WT B6 mice,and analyzed mononuclear cell infiltration,muscle autoantigens expression,and the intramuscular CD8~+ T cells infiltration and MHC-I expression on the surface of new myofiber in TA muscle on day 15,30 and 60 days post-injection.Data showed that PNIPAM hydrogel could induce a skeletal muscle inflammation during 2 months.The inflammation response in hydrogel injecting area reached a peak on day 30,and then gradually decreased.Interestingly,the mixture of PNIPAM hydrogel/C-protein initiated a more severe and persistent muscle inflammatory response,than hydrogel injection alone.Immunofluorecence analysis demonstrated that the hydrogel induced the chronic muscle inflammation featured by the CD8~+ T cell infiltration and the increased class I MHC-positive regenerating myofibers.In conclusion,our work holds a new promise for preparing animal model of muscle inflammation using hydrogel as a bio-driver.And hydrogel-induced myositis model maybe useful for investigating pathologic mechanisms of muscle injury,inflammation and regeneration.
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