Effect of Xingnao Yizhi Prescription on Sex Hormone Signaling in Diabetic Dementia Mice
Author(s): GAO Xiao-fei, XU Meng, ZHANG Jing, YUE Ren-song, Chengdu University of Traditional Chinese Medicine, Teaching Hospital of Chengdu University of TCM
Pages: 81-85
Year: 2017 Issue: 2
Journal: Chinese Journal of Experimental Traditional Medical Formulae
Keyword: Xingnao Yizhi prescription; diabetic dementia; plasma sex hormone level; estrogen receptor α(Erα); estrogen receptor β(Erβ);
Abstract: Objective: To investigate the effect and mechanism of Xingnao Yizhi prescription on mRNA expression levels of serum hormone and estrogen receptor( Erα and Erβ) for type 2 diabetic dementia mice.Method: Kunming mice were set as blank group. KK-AY mice with blood sugar ≥13. 9 mmol·L- 1were randomly divided into model group, piracetam group, and Xingnao Yizhi prescription group. Piracetam tablets( 150mg·kg- 1) were given by ig administration in piracetam group. Xingnao Yizhi prescription( 7. 2 g·kg- 1) was given by ig administration in Xingnao Yizhi prescription group. Equal volume of saline was given in normal group and model group by ig administration. Morris water maze test was conducted before and after drug administration,and latent period was recorded. Plasma,hippocampus samples and frontal cortex were collected as soon as possible after anesthesia in mice. Enzyme linked immunosorbent assay( ELISA) was used to test plasma sex hormone level,such as follicle-stimulating hormone( FSH),progesterone( P),estradiol( E2),and testosterone( T). Real-time PCR was used to test Erα,Erβ mRNA expression levels. Result: As compared with model group,the latent period was significantly shortened in blank group,piracetam group,and Xingnao Yizhi prescription group( P < 0. 05); LH and T levels were significantly reduced in blank group and Xingnao Yizhi prescription group( P < 0. 05),and E2 and P levels were significantly increased( P < 0. 05). As compared with piracetam group,LH level was significantly reduced in Xingnao Yizhi prescription group( P < 0. 05). As compared with the model group,Erβ mRNA expression level was significantly increased in piracetam group and Xingnao Yizhi prescription group( P < 0. 05). Conclusion:The emergence of diabetic dementia may be related to the sex hormone pathway. Xingnao Yizhi prescription can improve the mild cognitive impairment in patients with diabetes mellitus,and the mechanism may be associated with improving plasma E2,P levels and Erβ mRNA expression levels,and reducing LH and T levels.
Pages: 81-85
Year: 2017 Issue: 2
Journal: Chinese Journal of Experimental Traditional Medical Formulae
Keyword: Xingnao Yizhi prescription; diabetic dementia; plasma sex hormone level; estrogen receptor α(Erα); estrogen receptor β(Erβ);
Abstract: Objective: To investigate the effect and mechanism of Xingnao Yizhi prescription on mRNA expression levels of serum hormone and estrogen receptor( Erα and Erβ) for type 2 diabetic dementia mice.Method: Kunming mice were set as blank group. KK-AY mice with blood sugar ≥13. 9 mmol·L- 1were randomly divided into model group, piracetam group, and Xingnao Yizhi prescription group. Piracetam tablets( 150mg·kg- 1) were given by ig administration in piracetam group. Xingnao Yizhi prescription( 7. 2 g·kg- 1) was given by ig administration in Xingnao Yizhi prescription group. Equal volume of saline was given in normal group and model group by ig administration. Morris water maze test was conducted before and after drug administration,and latent period was recorded. Plasma,hippocampus samples and frontal cortex were collected as soon as possible after anesthesia in mice. Enzyme linked immunosorbent assay( ELISA) was used to test plasma sex hormone level,such as follicle-stimulating hormone( FSH),progesterone( P),estradiol( E2),and testosterone( T). Real-time PCR was used to test Erα,Erβ mRNA expression levels. Result: As compared with model group,the latent period was significantly shortened in blank group,piracetam group,and Xingnao Yizhi prescription group( P < 0. 05); LH and T levels were significantly reduced in blank group and Xingnao Yizhi prescription group( P < 0. 05),and E2 and P levels were significantly increased( P < 0. 05). As compared with piracetam group,LH level was significantly reduced in Xingnao Yizhi prescription group( P < 0. 05). As compared with the model group,Erβ mRNA expression level was significantly increased in piracetam group and Xingnao Yizhi prescription group( P < 0. 05). Conclusion:The emergence of diabetic dementia may be related to the sex hormone pathway. Xingnao Yizhi prescription can improve the mild cognitive impairment in patients with diabetes mellitus,and the mechanism may be associated with improving plasma E2,P levels and Erβ mRNA expression levels,and reducing LH and T levels.
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