Author(s): CAO Yi, WANG Chao-qun, XU Feng, JIA Xiu-hong, LIU Guang-xue, YANG Sheng-chao, LONG Guang-qiang, CHEN Zhong-jian, WEI Fu-zhou, YANG Shao-zhou, FUKUDA Kozo, WANG Xuan, CAI Shao-qing, Department of Chemical Biology, School of Pharmaceutical Sciences, Peking University, State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Yunnan Provincial Research Center on Good Agricultural Practice for Dominant Chinese Medicinal Materials, Yunnan Agricultural University, WenshanS amqi Research Institute, MiaoxiangSanqi Technology Company, Uchida Wakanyaku Ltd.
Pages: 3773-3781
Year: 2016 Issue:  20
Journal: China Journal of Chinese Materia Medica
Keyword:  Panax notoginseng; semimicro and highly efficient extraction method; continuous cropping obstacles; LC-MS-IT-TOF; HPLC fingerprint; characteristic peaks; notoginsenoside K/notoginsenoside Fa;
Abstract: Panax notoginseng is a commonly used traditional Chinese medicine with blood activating effect while has continuous cropping obstacle problem in planting process. In present study,a semimicroextraction method with water-saturated n-butanol on 0. 1 g notoginseng sample was established with good repeatability( RSD < 2. 5 %) and 9. 6 %-20. 6 % higher extraction efficiency of seven saponins than the conventional method. A total of 16 characteristic peaks were identified by LC-MS-IT-TOF,including eight 20( S)-protopanaxatriol( PPT) type saponins and eight 20( S)-protopanaxadiol( PPD) type saponins. The established method was utilized to evaluate the quality of notoginseng samples cultivated by manual intervened methods to overcome continuous cropping obstacles. As a result,HPLC fingerprint similarity,content of Fa and ratio of notoginsenoside K and notoginsenoside Fa( N-K / Fa) were found out to be as valuatable markers of the quality of samples in continuous cropping obstacle research,of which N-K / Fa could also be applied to the analysis of notoginseng samples with different growth years. Notoginseng samples with continuous cropping obstacle had HPLC fingerprint similarity lower than 0. 87,in consistent with normal sample,and had significant lower content of notoginsenoside Fa and significant higher N-K / Fa( 2. 35-4. 74) than normal group( 0. 45-1. 33). All samples in the first group with manual intervention showed high similarity with normal group( > 0. 87),similar content of common peaks and N-K / Fa( 0. 42-2. 06). The content of notoginsenoside K in the second group with manual intervention was higher than normal group. All samples except two displayed similarity higher than 0. 87 and possessed content of 16 saponins close to normal group. The result showed that notoginseng samples with continuous cropping obstacle had lower quality than normal sample. And manual intervened methods could improve their quality in different levels. The method established in this study was simple,fast and accurate,and the markers may provide new guides for quality control in continuous cropping obstacle research of notoginseng.