Author(s): ZHI Xiaoping, CUI Panpan, LIU Yingxia
Pages: 341-345
Year: 2016 Issue:  4
Journal: Journal of Nanchang University(Natural Science)
Keyword:  HPLC-MS/MS; Urine samples; Tyrosine; Tryptophan;
Abstract: A new method for the simultaneous determination of free tyrosine and tryptophan in human urine samples by HPLC-MS/MS was developed.The chromatographic separation was achieved on an Agilent XDB-C18 column(4.6×50 mm,1.8μm),the column temperature was 25 ℃,the mobile phase was CH3CN-0.1%HCOOH(10:90,v/v),the flow rate was 0.3 mL·min-1 .In the full scan mode,the m/z 182.2 and m/z 205.2 were identified as the precursor ions of tyrosine and tryptophan,the m/z 136.1 and m/z 188 were quantitative product ion,respectively.The analysis was realized by multiple reaction-monitoring modes.Under optimal conditions,the linear ranges of tyrosine and tryptophan were 0.001-2.00μg · mL-1 ,and the limits of detection were 0.10 and 0.25 ng·mL-1 ,respectively.The average recoveries for tyrosine and tryptophan(spiked at three different levels)ranged from 74.0 to 124.0%,the RSD was below 7.97%.The method is simple,rapid and sensitive,and suitable for the simultaneous determination of free tyrosine and tryptophan in human urine.