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METHOD OF EXTRACTION AND PURIFICATION OF CYTOKININS IN LAMINARIA JAPONICA
Author(s): 
Pages: 509-514
Year: Issue:  5
Journal: OCEANOLOGIA ET LIMNOLOGIA SINICA

Keyword:  海带细胞激动素纯化高效液相色谱;
Abstract: 海带样品于2001年采自青岛太平角海湾,用三种不同方法处理样品,通过对实验方法及条件的筛选,确立了提取、纯化和分离海带中细胞激动素的方法步骤和流程.结果表明,最佳方法的基本步骤为:(1) 用甲醇∶水∶醋酸(70 ∶ 30 ∶ 3, V/V/V)从海带组织中提取出细胞激动素,再用PVPP柱去除提取液中的酚类和其他杂质;(2) 将提取液通过连在一起的PVPP柱、DEAE柱和C18柱,以纯化样品并同时从自由基、核苷、核苷酸和葡糖苷型细胞激动素混合物中分离出核苷酸型细胞激动素;(3) 用接有氨基柱的正相液相色谱进一步将葡糖苷型细胞激动素分离出来;(4) 用C18液相色谱柱分离其他各种细胞激动素.
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