High-fat diet alters saccharin preference and dopamine and opioid related gene expression in female rats
Author(s): SUN Bo, SONG Lin, LUO Xiao, MENG Kai, YAN Jian-qun, Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Xi’an Jiaotong University Health Science Center
Pages: 783-788
Year: 2016 Issue: 6
Journal: Journal of Xi'an Jiaotong University(Medical Sciences)
Keyword: high-fat diet; saccharin preference ratio; dopamine; opioid ligands and receptors; reward system;
Abstract: Objective To determine whether high-fat(HF)diet affects preference for saccharin solutions in female rats and the possible underlying mechanisms.Methods Adult female Sprague-Dawley rats were divided into2 groups:CHOW group was fed with a normal chow diet(13.0% calories from fat)and HF group with an HF diet(50.5% calories from fat).At the end of the 9th week,we measured body weight gain and plasma leptin levels,saccharin intake and preference using a two-bottle preference test,and dopamine and opioid related gene expression in reward-related nuclei and hypothalamus using real-time PCR.Results Compared with CHOW group,HF group had greater body weight gain and higher plasma leptin levels.The consumption and preference ratio for 0.001 mol/L saccharin were lower in the HF rats,while the consumption and preference ratios for 0.02 and0.04 mol/L saccharin were higher in these rats.HF group had higher dopamine reuptake transporter(DAT)mRNA expression,lower dopamine receptor D1,dopamine receptor D2 and DARPP-32 dopamine-and cAMP-regulated phosphoprotein mRNA expression,and lowerμ-opioid receptor(MOR)mRNA expression in reward-related nuclei.ConclusionHF diet affects preference for saccharin solution in female rats,and it may be partially due to the state of reward hypofunction induced by alterations of dopamine and opioid system in reward-related nuclei.
Pages: 783-788
Year: 2016 Issue: 6
Journal: Journal of Xi'an Jiaotong University(Medical Sciences)
Keyword: high-fat diet; saccharin preference ratio; dopamine; opioid ligands and receptors; reward system;
Abstract: Objective To determine whether high-fat(HF)diet affects preference for saccharin solutions in female rats and the possible underlying mechanisms.Methods Adult female Sprague-Dawley rats were divided into2 groups:CHOW group was fed with a normal chow diet(13.0% calories from fat)and HF group with an HF diet(50.5% calories from fat).At the end of the 9th week,we measured body weight gain and plasma leptin levels,saccharin intake and preference using a two-bottle preference test,and dopamine and opioid related gene expression in reward-related nuclei and hypothalamus using real-time PCR.Results Compared with CHOW group,HF group had greater body weight gain and higher plasma leptin levels.The consumption and preference ratio for 0.001 mol/L saccharin were lower in the HF rats,while the consumption and preference ratios for 0.02 and0.04 mol/L saccharin were higher in these rats.HF group had higher dopamine reuptake transporter(DAT)mRNA expression,lower dopamine receptor D1,dopamine receptor D2 and DARPP-32 dopamine-and cAMP-regulated phosphoprotein mRNA expression,and lowerμ-opioid receptor(MOR)mRNA expression in reward-related nuclei.ConclusionHF diet affects preference for saccharin solution in female rats,and it may be partially due to the state of reward hypofunction induced by alterations of dopamine and opioid system in reward-related nuclei.
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