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huan yuan xing gu zuo gan zuo gsh zai xiao shu ti wai shou jing zhong de zuo yong chu tan
Author(s): 
Pages: 1-2
Year: Issue:  6
Journal: Zhejiang Journal Animal Science and Veterinary Medicine

Keyword:  还原型谷胱甘肽体外受精受精率产仔率小鼠;
Abstract: 为研究还原型谷胱甘肽(GSH)在小鼠体外受精中的作用,试验选用C57BL/6J小鼠(雄鼠12周龄,雌鼠4周龄)、ICR受体鼠(8-10周龄)。GSH添加浓度分别为终浓度0、1 mmol/L、2 mmol/L,每组超排雌鼠5只,转基因小鼠净化试验每个品系超排雌鼠10只。结果显示,在受精液(HTF)中添加GSH 1 mmol/L时,小鼠体外受精效果最好,受精率达70.8%,胚胎形状正常,未受精单细胞与异形胚较少,胚胎移植平均妊娠率达93.5%。
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