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Studies on the Purification and Characteristics of Urease in Dactylosporangium aurantiacum in Paddy Fields
Author(s): 
Pages: 595-598
Year: Issue:  5
Journal: ACTA AGRICULTURAE UNIVERSITATIS JIANGXIENSIS(NATURAL SCIENCES EDITION)

Keyword:  稻田Dactylosporangium aurantiacum脲酶;
Abstract: 经磷缓液提取,乙醇沉淀,超滤去杂,然后经CM-纤维素柱层析,DEAE-SephadexA-50柱层析纯化了Dactylosporangium aurantiacum菌脲酶,纯化倍数达50.01,活力回收率为17.81%.经SDS-PAGE垂直平板电泳测得其分子量为42 000.经氨基酸组成分析,该酶含271个氨基酸残基,该酶的紫外吸收峰为275 nm.
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